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KMID : 0380020170320040286
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 4 p.286 ~ p.292
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Optimization of Alkail Extraction for Production of Protein Concentrates from Lipid Extracted Algae
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Sin Seul-Gi
Jo Jae-Min
Jung Hyun-Jin
Seo Joo-Hyun
Kim Jin-Woo
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Abstract
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In this study, an attempt was made to optimize protein extraction from lipid extracted microalgae (LEA) by alkali solution. To enhance the productivity, extraction solvent, extraction pH, temperature, time and precipitation pH were evaluated to maximize the protein extraction from defatted Tetraselmis KCTC 12236BP. Independent variables tested were organic solvents, extraction pH (5-13), precipitation pH (2.3-4.0), extraction time (2.6-9.4 h) and extraction temperature (26-94°C). Alkali solution (2 mol NaOH) was found to be effective in extraction evaluating, the impact of the solvents on protein extraction. The results showed that the extraction pH was an influencing variable and extraction yield increased both with increase in extraction temperature time and precipitation pH. The yield of protein showed a maximum level at temperature of 64°C and precipitation pH of 3.38, respectively. Under the optimal condition, LEA protein yield reached 71.7% as compared to 34.4% with the initial condition with 50°C, 30 min of agitation and 1 h of precipitation, representing a 2.1 fold increase in protein yield.
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KEYWORD
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lipid extracted microalgae, protein extraction, aliki extraction, optimization, animal feed
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